Microbial biofilms have been playing a major role in many processes such as infection, disease spreading and resistance to antibiotics.
Uncontrolled microbial contamination is a common cause of rejection of biomedical devices such as plastic catheters, prosthetic implants and medical devices.
  • High resolution imaging of the microbial surface using SEM helps better understanding of the morphology of microbial populations, bacteria communication and the biofilm formation.
  • TESCAN SEMs help researchers to visualize microbial populations with great focal depth and high resolution.
  • The microbial biofilms can be observed non coated with the environment-like conditions either using our variable pressure UniVac mode or using the Cryo-SEM adaptation.
  • For scientists interested in the ultrastructure features of individual, uncoated bacteria, we offer ultrahigh resolution SEM MAIA3, which is specifically designed to provide the unprecedented sub-nanometre resolution at very low accelerating voltages.
Detailed view of Streptococcus mutans

Related Application Notes

Adhesion of Streptococcus mutans to Dental Restorative Materials
Development of early childhood caries (ECC) is associated with the colonization of the oral cavity by oral microbes. The disease is characterized by severe decay of infant’s teeth. The colonization starts soon after the birth and its diversity increases later on, with the eruption of primary teeth. While external factors such as feeding habits, sugar expose and hereditary factors support development of the ECC, the onset and progression also greatly depends on the composition of microflora in the mouth cavity.
pdf – 1.1 MB
STEM detector in life science applications
Scanning transmission electron microscopy (STEM) has become a highly effective, easy-to-use technique for imaging biological thin sections (lamellae) in SEM. Multiple sample observation, automated stage navigation, and ultra-high resolution imaging make this technique an attractive solution for high-contrast observation of TEM sections with excellent results, and minimal operator’s time.
pdf – 2.4 MB
Low temperature scanning electron microscopy for Life Sciences
Low temperature scanning electron microscopy (Cryo-SEM) has become an established technique for capturing and observing biological samples close to their natural state. It is a method of choice, where the traditional sample preparation (e.g. critical point drying) causes unwanted changes in the sample structure. A Cryo-SEM workflow typically involves sample fixation using either flash-freezing in a liquid nitrogen slush or high-pressure freezing. The frozen samples are then transferred under vacuum to a cryo sputter coater, where they are coated with a conductive layer of metals or carbon. Finally, the samples are inserted into a SEM chamber equipped with a cryo-stage and observed in high vacuum environment.
pdf – 4.8 MB
Q-PHASE Live Cell Imaging
Q-PHASE, the multimodal holographic microscope, is a unique instrument for quantitative phase imaging (QPI). The main application of this technique is in live cell imaging where advantages such as no need for labeling, low phototoxicity, easy segmentation, cell dry mass interpretation of measured signal and suitability for long term experiments are used. Q-PHASE is built as a transmitted light microscope in an inverted configuration for easy handling with biological samples. Appropriate conditions for live cells are ensured by the microscope incubator heated to 37°C and low exposures of light for QPI. Moreover, there is no need for specific sample preparation. The cells are just seeded into a suitable observation chamber and examined.
pdf – 1.7 MB