In this application example, a workflow to microcharacterize (in 2D and 3D), fabricate micropillars and perform in-situ nanoindentation testing to investigate the mechanical properties of beechwood using one single instrument is presented. The TESCAN RISE (FIB-SEM) is able to produce ultrastructural images that can be correlated with chemical maps generated by Raman microscopy that clearly show how the composition and structure relate to one another. Furthermore, the same platform can be used to prepare micropillars at site-specific locations to perform in-situ mechanical testing through nanoindentation. All these datasets can be easily correlated to see the relationships between structure, composition and mechanical properties. The workflow presented here can be applied to study a range of other organic and inorganic materials.
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- Workflow in the TESCAN RISE FIB-SEM microscope designed for investigation of natural materials.