Microbiologia

Biofilmes microbianos têm desempenhado um papel importante em diversos processos, tais como infecção, doenças contaminantes e resistência a antibióticos.
Contaminação microbiana descontrolada é uma causa comum de rejeição de dispositivos biomédicos, tais como cateteres de plástico, implantes de próteses e dispositivos médicos.
  • Imagens de alta resolução da superfície microbiana utilizando MEV ajuda a melhor compreensão da morfologia de populações microbianas, comunicação de bactérias e a formação de biofilmes.
  • Os MEVs da TESCAN ajudam os investigadores a visualizar as populações microbianas com grande profundidade focal e alta resolução.
  • Os biofilmes microbianos podem ser observados não revestidos com as condições ambientais, utilizando o nosso modo UNIVAC de pressão variável ou utilizando a solução MEV com criogenia
  • Para os cientistas interessados nas características da ultraestrutura das bactérias individuais, não revestidas, oferecemos o MEV-FEG MAIA de ultra resolução, que é projetado especificamente para fornecer a resolução sub nanométrica sem precedentes em tensões de aceleração muito baixas.
Microbiologia
Vista detalhada de Streptococcus mutants

Nota de Aplicações Relacionadas

Adhesion of Streptococcus mutans to Dental Restorative Materials
Development of early childhood caries (ECC) is associated with the colonization of the oral cavity by oral microbes. The disease is characterized by severe decay of infant’s teeth. The colonization starts soon after the birth and its diversity increases later on, with the eruption of primary teeth. While external factors such as feeding habits, sugar expose and hereditary factors support development of the ECC, the onset and progression also greatly depends on the composition of microflora in the mouth cavity.
pdf – 1,1 MB
STEM detector in life science applications
Scanning transmission electron microscopy (STEM) has become a highly effective, easy-to-use technique for imaging biological thin sections (lamellae) in SEM. Multiple sample observation, automated stage navigation, and ultra-high resolution imaging make this technique an attractive solution for high-contrast observation of TEM sections with excellent results, and minimal operator’s time.
pdf – 2,4 MB
Low temperature scanning electron microscopy for Life Sciences
Low temperature scanning electron microscopy (Cryo-SEM) has become an established technique for capturing and observing biological samples close to their natural state. It is a method of choice, where the traditional sample preparation (e.g. critical point drying) causes unwanted changes in the sample structure. A Cryo-SEM workflow typically involves sample fixation using either flash-freezing in a liquid nitrogen slush or high-pressure freezing. The frozen samples are then transferred under vacuum to a cryo sputter coater, where they are coated with a conductive layer of metals or carbon. Finally, the samples are inserted into a SEM chamber equipped with a cryo-stage and observed in high vacuum environment.
pdf – 4,8 MB
Q-PHASE Live Cell Imaging
Q-PHASE, the multimodal holographic microscope, is a unique instrument for quantitative phase imaging (QPI). The main application of this technique is in live cell imaging where advantages such as no need for labeling, low phototoxicity, easy segmentation, cell dry mass interpretation of measured signal and suitability for long term experiments are used. Q-PHASE is built as a transmitted light microscope in an inverted configuration for easy handling with biological samples. Appropriate conditions for live cells are ensured by the microscope incubator heated to 37°C and low exposures of light for QPI. Moreover, there is no need for specific sample preparation. The cells are just seeded into a suitable observation chamber and examined.
pdf – 1,7 MB