Detection of QD- immunolabelled sections prepared on glass slides

TESCAN UHR SEM MAIA provides enhanced contrast
In this application example, the ultra-high resolution (UHR) scanning electron microscope (SEM) MAIA equipped with the Triglav™ column and the Mid-Angle BSE detector optimised for low beam energies has been used to localize and image quantum dots (QD) - immunolabelled thin sections prepared on glass slides. This is due to the enhanced contrast and the excellent performance at low beam energies needed to detect QD probes. The results show here, can be useful to research in tumour cells and in other cell biology research.

Documents for download

Visualization of the QD- immunostained somatostatin hormone using an ultra-high resolution TESCAN MAIA3 FE-SEM
Somatostatinoma is a type of a neuroendocrine tumor that typically develops in the region of the duodenum or pancreas. This kind of tumor is characterized by an excessive somatostatin hormone secretion. These elevated levels of somatostatin can be used for investigation of the somatostatin tumor cells, typically by the positive immunohistochemical staining. Correlative light-electron microscopy imaging has become a very popular approach due to the usage of quantum-dotbased immunoprobes, which can be inherently detected by both techniques.
pdf – 1.2 MB