FIB-SEM Tomography of Biological Specimens with the new TESCAN S9000G

Nov/29/2018
Advanced Focused Ion Beam-Scanning Electron Microscopy for 3D ultrastructural analysis of mitochondria-ER interactions in mammalian cells
In this application example, we demonstrate by using the new TESCAN S9000G microscope, that FIB-SEM technology is an innovative approach to study and resolve the complexity of subcellular environment. The advancement in 3D imaging allows the precise morphological description of individual organelles and identification of their mutual connections. Given the reliability, efficiency, and high resolution of FIB-SEM technology, this analysis represents an important foundation to better understand organelle cross-talk in mammalian cells.

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3D ultrastructural reconstruction of studied mammalian cell. Selected FIB-SEM stacks show intracellular organization with clear contacts between organelles.
3D ultrastructural reconstruction of studied mammalian cell. Selected FIB-SEM stacks show intracellular organization with clear contacts between organelles.


Application Example

Advanced Focused Ion Beam-Scanning Electron Microscopy for 3D ultrastructural analysis of mitochondria-ER interactions in mammalian cells
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