Large-volume 3D structural studies of biological specimens using the TESCAN S9000X Xe plasma FIB

Mar/14/2019
3D Tomography of Zebrafish Embryos Examined with Xe Plasma FIB
In this application example, we show how the TESCAN Xe plasma FIB is suitable to performing large-volume FIB-SEM tomography of biological specimens offering higher resolution compared to conventional techniques such as CT, allowing the visualization and identification of specific features, organs and micro- and nanostructures. In particular, we demonstrate the viability of this technique by presenting a FIB-SEM tomography of a Zebrafish embryo, an important specimen for biological research.

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3D reconstruction made by stacking the individual slices.
3D reconstruction made by stacking the individual slices.


Application Example

3D Tomography of Zebrafish Embryos Examined with Xe Plasma FIB
Zebrafish (Danio rerio) are a type of freshwater fish that are extremely important to biological research, in particular genetics and diseases. Reasons for their widespread include:
  • They have a similar genetic makeup to humans sharing 70% of their genes
  • 84% of genes associated with human diseases have a zebrafish counterparts
  • Their embryos are almost transparent making it easy to observe the development of internal structures
  • Eggs are fertilised and develop outside the mother’s body making it easy to study early development
  • They have the same major organs and tissues as humans e.g. muscle, blood, kidney and eyes
  • They grow extremely fast, developing as much in a day as a human embryo develops in a month
  • Cheaper to keep than mice
  • Breed prolifically
pdf – 6.1 MB
TESCAN S9000X
TESCAN S9000X