Applications

  • Cell biology
  • Cancer research
  • Drug testing
  • Cell death analyses
  • Biocompatibility testing
  • Cell cycle analyses
  • Stem cell research
  • Plant biology and hydrobiology
  • Imaging cells in 3D environment

Metastatic cancer cell – immune cell interaction (T-cell invading polyploid cancer cell)

Formation of polyploid ovarian cancer cell A2780

Human embryonal stem cells: Colony formation

Diversity of metastatic cancer cells population (PC-3 cells)

Communication of human chondrogenic cells

Sperm cells movement for semen analysis

Intake of apodox and treatment response of human breast cancer cell line MDA-MB-231

Intercellular communication of human metastatic polyploid prostate cancer cell PC-3

Observation of HEK293 cell population transfected with GFP-Vesicular Stomatis Virus Coat Protein

5 days of human embryonal stem cells (HESC) colony life

Application notes

CANCER RESEARCH
Q-PHASE allows observation of cell reactions to different treatments without any labeling. This innovative approach opens up new opportunities in cancer research. Through experiments, researchers look at how cancer cells behave and try to understand cancer at its deepest levels. Data from experiments can help researchers figure out what controls cell division and cell death, find out what makes cancer cells spread or metastasize, identify unique characteristics of cancer cells to design new therapeutic strategies, and find out why certain cancer cells become resistant to therapy. Additional fluorescence data provides the possibility to simultaneously verify observed processes using a single instrument.
pdf – 1.3 MB
LIVE CELL IMAGING
Q-PHASE is a unique instrument dedicated to live cell imaging where advantages such as no need for labeling, low phototoxicity, easy segmentation, cell dry mass interpretation of measured signal, and suitability for long term experiments are used. Q-PHASE is built as a transmitted light microscope in an inverted configuration for easy handling with biological samples. Appropriate conditions for live cells are ensured by the microscope incubator and low exposures of light. Moreover, there is no need for specific sample preparation. The cells are just seeded into a suitable observation chamber and examined.
pdf – 1.7 MB
NON-INVASIVE AND LABEL-FREE DETECTION OF CELL DEATH
Q-PHASE enables easy and fast detection of specific cell death without any need for labeling nor additional damage to cells. Thanks to the quantitative phase imaging and advanced analysis software, Q-PHASE provides pre­cise information on morphological changes and dynamics of individual cells during cell death processes. Data obtained by Q-PHASE could help researches not only to distinguish between different types of cell death, but also to assess the rate of cell reactions to drug treatment or other stress conditions, and answer the questions of cancer cell behavior.
pdf – 1.6 MB

Publications


S. Dostalova, et al.: Prostate-Specific Membrane Antigen-Targeted Site-Directed Antibody-Conjugated Apoferritin Nanovehicle Favorably Influences In Vivo Side Effects of Doxorubicin, Scientific Reports 8:8867, 2018. PDF

S. Salucci, et al.: Marine bisindole alkaloid: A potential apoptotic inducer in human cancer cells, European Journal of Histochemistry 62(2), 2018. PDF

L. Pastorek, et al.: Holography microscopy as an artifact-free alternative to phase-contrast, Histochem Cell Biol. 149(2), 2018.
 
B. Gal, et al.: Distinctive behaviour of live biopsy-derived carcinoma cells unveiled using coherence-controlled holographic microscopy, PLoS One 12(8), 2017. PDF
 
L. Strbkova, et al.: Automated classification of cell morphology by coherence-controlled holographic microscopy, J. Biomed. Opt. 22(8), 2017. PDF

J. Babocky, et al.: Quantitative 3D Phase Imaging of Plasmonic Metasurfaces, ACS Photonics 4(6), 2017.

J. Zemanova, et al.: Chk1 inhibition significantly potentiates activity of nucleoside analogs in TP53-mutated B-lymphoid cells, Oncotarget 7(38), 2016. PDF
 
L. Strbkova, et al.: The adhesion of normal human dermal fibroblasts to the cyclopropylamine plasma polymers studied by holographic microscopy, Surface and Coatings Technology 295, 2016. PDF
 
J. Balvan, et al.: Oxidative Stress Resistance in Metastatic Prostate Cancer: Renewal by Self-Eating, PLoS One 10(12), 2015. PDF
 
J. Collakova, et al.: Coherence-controlled holographic microscopy enabled recognition of necrosis as the mechanism of cancer cells death after exposure to cytopathic turbid emulsion, J. Biomed. Opt. 20(11), 2015. PDF
 
V. Kollarova, et al.: Quantitative phase imaging through scattering media by means of coherence-controlled holographic microscope, J. Biomed. Opt. 20(11), 2015. PDF
 
A. Krizova, et al.: Dynamic phase differences based on quantitative phase imaging for the objective evaluation of cell behavior, J. Biomed. Opt. 20(11), 2015.
 
J. Balvan, et al.: Multimodal Holographic Microscopy: Distinction between Apoptosis and Oncosis, PloS One 10(3), 2015. PDF
 
M. Lostak, et al.: Coherence-controlled holographic microscopy in diffuse media, Opt. Express 22(4), 2014. PDF
 
T. Slaby, et al.: Off-axis setup taking full advantage of incoherent illumination in coherence-controlled holographic microscope, Opt. Express 21(12), 2013. PDF
 
H. Janeckova, et al.: Proving Tumour Cells by Acute Nutritional/Energy Deprivation as a Survival Threat: A Task for Microscopy, Anticancer Res. 29(6), 2009. PDF