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TESCAN Volume SEM

Versatile SEM for 2D and 3D characterization of your biological samples

Volume SEM methods provide insights into biological material in high resolution that allows researchers to comprehensively understand the internal structure and functioning of biological systems in 3D. 

The 2D imaging capability of almost any SEM can be expanded to a 3rd dimension with specialized software or hardware. Method of choice depends on the priority and character of the sample. Samples that have been sectioned beforehand, collected on a substrate as an array of serial sections (Array Tomography) can be automatically scanned with a SEM to produce a 3D stack of images. This method allows repeated scanning and the ability to go back to additional regions of interest at any time. On the other hand, a SEM equipped with a special in-chamber microtome is able to analyze volumes of exceptional dimensions in the field electron microscopy – blocks of almost one cubic millimeter can be examined.This allows us to understand the complex structural organization of cells, tissues, organs and organisms, and relationships over a large distance, such as animal connectomes.

Microscope flexibility, throughput and ease of use are of utmost importance for instruments in core facilities and imaging centers. Therefore, TESCAN volume SEMs are designed to be fully operational after only a short training session. This is possible with our modular Essence™ GUI that is configurable and predefined for specific applications. Quality of results is assured by streamlining the process from easy acquisition setup through to data processing, machine-learning assisted segmentation and visualization in a single, unified TESCAN software environment. 

In-chamber microtome slicing & imaging (SBF-SEM)

Serial Block Face – SEM (SBF-SEM) utilizes an in-chamber microtome to allow large volume analysis by in-situ sectioning and imaging of samples.  Samples are usually resin-embedded blocks of cells, tissues or small organisms. SBF-SEM is used for larger volumes as it enables slicing of areas as wide as 1mm enabling multiple regions of interest to be acquired during the run. Precise microtome slicing through the sample together with an efficient detection system allows for the acquisition of large datasets with a modest Z resolution in a reasonable time with swift reconfiguration back to a normal SEM regime when the acquisition is complete. 

 Array Tomography
 

In Array Tomography, a resin-embedded tissue specimen is cut into ultrathin sections which are then bonded to a silicon wafer outside the SEM chamber. The resulting array is scanned automatically with a dedicated software module. This approach is especially beneficial when the sample needs to be preserved for future reference or for immuno-labelling of sections. 

This method does not require any specialized hardware to be installed on the SEM. Streamlined software guides the user through the acquisition setting in a simple and intuitive manner and also offers a straightforward way of processing the resulting data to create 3D visualizations.

  • Extend your SEM for to perform Volume SEM analysis Integrated Serial Block-Face SEM (SBF-SEM) in-chamber microtome and Array Tomography software module allow large volume analysis of samples
  • Obtain greater contrast from your biosamples. Use dedicated low energy BSE detector optimized for volume SEM application
  • Speed up your data acquisition Highly efficient detection system shortens the time of analysis and increases throughput

  • Acquire multiple regions of interest easily. SBEMimage is an open-source Python application to run acquisitions with serial block-face imaging allowing advanced multi-ROI analysis
  • Visualise your data intuitively without effort. Import, process and visualize 3D image data with TESCAN Volume Analysis software. This simple but powerful three-dimensional data processing tool utilizes guided, optimized workflows for quick import, processing, AI assisted segmentation and visualizing your 3D datasets
  • Enjoy high flexibility and usability of your microscope. The unmatched versatility of TESCAN SEMs fits the needs of most users in core facilities or other research institutes

TESCAN Webinars:

SEM Large-volume Analysis in Life Sciences assisted by Serial Block-Face Imaging

  • TESCAN Serial Block Face Imaging solution for Life Sciences

    Product Flyer

    pdf 310 KB Download
  • TESCAN Esscence 3D Collision Model

    Product Flyer

    pdf 215 KB Download
Get in touch

Do you wish to discuss TESCAN VolumeSEM solution? Contact our Team directly!