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Cryo-TEM Lamella Preparation from Biological Samples

19 May 2021 (9 AM CET & 7 PM CET) 

ENGLISH

Low-temperature electron microscopy (cryo-EM) has become an established technique for capturing and observing beam-sensitive samples in their close-to-natural state. 

Cryo-sectioning is a standard method used for thinning and slicing such samples. However, the integrity of the specimens can be degraded easily by common artifacts such as knife marks, compression, or crevasses.  Focused ion beam (FIB), on the other hand, is used extensively to reveal sub-surface information from bulk material, create samples for other analytical modalities, or prepare ultra-thin specimens for analysis in transmission electron microscopy (TEM), without knife-sectioning artifacts. 

Moreover, combining the site-specific milling capabilities of FIB with scanning electron microscopy (SEM) opens a broad range of possibilities for preparing biological samples. FIB-SEM systems are widely used not only for routine preparation of ultra-thin TEM specimens but also for precision cross-sectioning and 3D volume imaging, which are possible in ambient temperatures as well as in cryogenic conditions. 

The seminar aims to demonstrate the feasibility and discuss the potential of TESCAN cryo-FIB-SEM as a reliable tool for cryo-TEM sample preparation, with its exceptional range of additional capabilities—all within a single versatile workstation.

About Ondrej Sulak

Ondřej Šulák received his Master’s degree in Analytical chemistry at Masaryk University in the Czech Republic. Then, he moved to France where he continued his Ph.D. studies and Postdoctoral research at Joseph Fourier University in Grenoble where he was focused on the structure-functional characterization of carbohydrate-binding proteins, mainly by X-ray crystallography, Electron Microscopy, SAXS, and other techniques. His industrial career started in the biotechnological company where he was responsible for the biochemistry department. In 2016, Ondrej joined TESCAN as Global Applications Director. Since January 2019 he works as Product Marketing Director for the Life Sciences segment.

Presenter: Ondrej Sulak
Date: 19 May 2021 (9 AM CET a 7 PM CET)