TESCAN Webinar:

SEM Large-volume Analysis in Life Sciences Assisted by Serial Block-Face Imaging

On demand

ENGLISH

We are excited to present the newest solution in our Life Sciences product family: Serial Block-Face Imaging (SBFI).

Serial Block-Face Imaging (also known as Serial Block-Face SEM) is a traditional technique for high-resolution structural analysis of the sample using an integrated microtome to the SEM chamber. The method uses a diamond knife of the microtome to cut a slice from the sample surface (20-100 nm thin) which is immediately imaged by SEM. This process is repeated to create an image stack allowing a reconstruction of the sample volume.

During this webinar we will discuss the key features that enable us to extend the capabilities of standard SEM to the volume scale of structural analysis, including:

  • in-chamber microtome device,
  • compact size,
  • simple and fast installation/de-installation,
  • SEM / FIB-SEM GM chamber compatibility,
  • effective LE-BSE imaging,
  • and/or TESCAN volume analysis software.

Key technical features of this exciting solution together with multiple application examples will be presented during this seminar.

3D volume reconstruction of resin-embedded flatworm (Platyhelminthes) sample. Dataset collected by TESCAN MIRA at 4 keV, 150 pA. Low energy BSE detector, inverted contrast. Low vacuum mode, 20 Pa, Pixel size 16 nm, Number of slices: 300, Volume acquired: 130 x 130 x 25 μm

Presenter: Ondrej Sulak

About Ondrej Sulak

Ondrej Sulak received his Master degree from Analytical chemistry at Masaryk University in the Czech Republic. Then, he moved to France where he continued his Ph.D. studies and Postdoctoral research at Joseph Fourier University in Grenoble where he was focused on the structure-functional characterization of carbohydrate-binding proteins, mainly by X-ray crystallography, Electron Microscopy, SAXS, and other techniques. His industrial career he started in the biotechnological company where he was responsible for biochemistry department. In 2016, Ondrej joint TESCAN as Global Applications Director. Since January 2019 he works as Product Marketing Director for Life Sciences segment.